Dr. Randy D. Reif
Assistant Professor of Chemistry

Photo of Dr. Randy D. Reif

Departments: Chemistry and Biochemistry
Office: Goodpaster Hall 156
Email: rdreif@smcm.edu
Phone: (240) 895-3202

Education:

Ph.D. Texas Tech, 2010.
B.A. St. Mary's College of Maryland, 2006.

Courses Taught:

  • Analytical Chemistry
  • General Chemistry I
  • General Chemistry II Lecture
  • Biochemistry I Lab
  • Contemporary Chemistry

Research/Professional Interests

Dr. Reif’s research interests are split between two major fields, cell analysis (bioanalytical chemistry) and RNA aptamer design and fabrication.  He is interested in designing new microfluidic devices designed to analyze mammalian cell samples by fluorescence microscopy and using these devices to study complex cellular processes such as (but not limited to) apoptosis.  One of the current projects in his lab focuses on the design and fabrication of a microfluidic device that incorporates glass surfaces on the top and bottom of a microfluidic channel but a gas-permeable polymer (PDMS) on the sides.  This device will potentially be compatible with both standard and inverted confocal microscopes while maintaining the health of the cells within the device during long-term analyses.

Dr. Reif is also interested in discovering new RNA aptamers that can be incorporated into RNA nanoparticles for a number of applications. He is currently working alongside Dr. Streu’s research group to design new RNA aptamers that bind drug molecules or protein targets using a technique called Systematic Evolution of Ligands by Exponential Enrichment (SELEX).  These RNA aptamers could be used for a variety of purposes including the design of therapeutic RNA nanoparticles, studying cell signaling, and/or influencing protein expression.  He is also interested in how RNA aptamers interact with large molecules such as cellular receptors or small molecular targets such as fluorogenic dyes.

Selected Publications

1. S.M. Burrows, R.D. Reif, D. Pappas, "Investigation of Photobleaching and Saturation of Single Molecules by Fluorophore Recrossing Events", Analytica Chimica Acta, 598, 135-142 (2007).

2.  D. Pappas, S.M. Burrows, R.D. Reif, "Exploring Biomolecular Interactions by Single Molecule Fluorescence Spectroscopy", TrAC: Trends in Analytical Chemistry, 26, 884-894 (2007).

3.  K. Liu, Y. Tian, S. Burrows, R.D. Reif, D. Pappas, “Mapping Hydrodynamic Vortices in Microfluidic Networks using Fluorescence Correlation Spectroscopy”, Analytica Chimica Acta, 651, 85-90 (2009).

4.  R.D. Reif, M.M. Martinez, K. Wang, D. Pappas, “Simultaneous Cell Capture and Induction of Apoptosis Using an anti-CD95 Affinity Microdevice”, Analytical and Bioanalytical Chemistry, 395, 787-795 (2009).

5.  R.D. Reif, K. Wang, D. Pappas, “Rapid Data Analysis Method of Differential Mobility Cytometry”, Analytical and Bioanalytical Chemistry, 395, 2411-2413 (2009).

6.  M.M. Martinez, R.D. Reif, D. Pappas, “Early Detection of Apoptosis in Living Cells by Fluorescence Correlation Spectroscopy,” Analytical and Bioanalytical Chemistry, 396, 1177-1185 (2010).

7.  R.D. Reif, C. Aguas, M.M. Martinez, D. Pappas, “Temporal Dynamics of Receptor-Induced Apoptosis in an Affinity Microdevice,” Analytical and Bioanalytical Chemistry, 397, 3387-3396 (2010).

8.  M.M. Martinez, R.D. Reif, and D. Pappas, “Detection of Apoptosis: A review of conventional and novel techniques,” Analytical Methods, 2, 996-1004 (2010).

9.  P. Guo, F. Haque, B. Hallahan, R. Reif, and H. Li, “Uniqueness, Advantages, Challenges, Solutions, and Perspectives in Therapeutics Applying RNA Nanotechnology,” Nucleic Acid Therapeutics, 22, 226-245 (2012).

10.  R. Reif, F. Haque, P. Guo, “Fluorogenic RNA Nanoparticles for Monitoring RNA Folding and Degradation in Real Time in Living Cells,” Nucleic Acid Therapeutics, (2012) In Press.

11.  R. Reif and P. Guo, “Design and Construction of RNA Nanoparticles Targeting Prostate Cancer,” Peixuan Guo (ed.) RNA Nanotechnology and Therapeutics, CRC Press. (2013) In Press.